AGO1 antibody

Catalog No. ABIN1831317

The Rabbit Monoclonal anti-AGO1 antibody (Clone 11C1028) (ABIN1831317) specifically detects AGO1 in WB, ELISA, IHC, IP, ICC, FACS and RIA. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for AGO1 antibody (ABIN1831317)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Radioimmunoassay (RIA)

Clone: 11C1028

Product Details anti-AGO1 Antibody

Specificity: Endogenous levels of total Argonaute 1 protein.

Purification: Purified

Immunogen: Synthetic peptide corresponding to the sequence around Ile392 of human Argonaute 1 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, ICC, IHC, IP, RIA, WB (1:1000)

Usage: Western Blot: Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, less than 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: AGO1 / EIF2C

Background: Name/Gene ID: AGO1

Synonyms: AGO1, Argonaute 1, EIF-2C 1, EIF2C1, GERP95, EIF2C, EIF2C 1, HAgo1, Q99, Argonaute1, Protein argonaute-1

Gene ID: 26523

UniProt: Q9UL18

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization