LIM Domain Binding 1 Protein antibody (AA 86-411)

Catalog No. ABIN1833095

The Rabbit Polyclonal anti-LIM Domain Binding 1 Protein antibody has been validated for WB, IHC, IP, IHC (p) and ICC. It is suitable to detect LIM Domain Binding 1 Protein in samples from Human and Mouse.

Quick Overview for LIM Domain Binding 1 Protein antibody (AA 86-411) (ABIN1833095)

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Binding 1 Protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-LIM Domain Binding 1 Protein Antibody

Binding Specificity: AA 86-411

Specificity: Human CLIM2 / LDB1

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 86 and 411 of LDB1 (SwissProt Q86U70). Percent identity by BLAST analysis: Human (100%), Rat (99%), Chicken, Xenopus (97%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), IP, WB (1:500 - 1:3000)

Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 20 % glycerol, 0.025 % proclin.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Keep as concentrated solution. Avoid multiple freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for LIM Domain Binding 1 Protein

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Alternative Name: CLIM2 / LDB1

Background: Name/Gene ID: LDB1

Synonyms: LDB1, CLIM-2, HLdb1, LIM domain-binding factor-1, LIM domain-binding protein 1, NLI, LIM domain binding 1, CLIM2, LDB-1, Nuclear LIM interactor

Gene ID: 8861

UniProt: Q86U70

Pathways: Stem Cell Maintenance, Chromatin Binding