LIM Domain Binding 1 Protein antibody (AA 303-352)

Catalog No. ABIN6736413

Product Details anti-LIM Domain Binding 1 Protein Antibody

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Binding Specificity: AA 303-352

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Guinea Pig, Horse, Rabbit, Chicken, Xenopus laevis, Pig, Bat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Binding 1 Protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human LDB1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa303-352 of human LDB1 (Q86U70-2, NP_003884). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Xenopus, Zebrafish (100%), Platypus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LIM Domain Binding 1 Protein

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Alternative Name: CLIM2 / LDB1 (LDB1 Products)

Synonyms: CLIM2 antibody, NLI antibody, chi antibody, clim2 antibody, nli antibody, xldb1 antibody, ldb1 antibody, LDB1 antibody, ldb4 antibody, ldb4b antibody, LIM domain binding 1 antibody, LIM domain binding 1 L homeolog antibody, LIM-domain binding 1b antibody, LIM domain binding protein 1 antibody, LIM domain binding 1a antibody, Ldb1 antibody, LDB1 antibody, ldb1.L antibody, ldb1b antibody, xldb1 antibody, ldb1a antibody

Background: Name/Gene ID: LDB1

Synonyms: LDB1, CLIM-2, HLdb1, LIM domain-binding factor-1, LIM domain-binding protein 1, NLI, LIM domain binding 1, CLIM2, LDB-1, Nuclear LIM interactor

Gene ID: 8861

NCBI Accession: NP_003884

UniProt: Q86U70

Pathways: Stem Cell Maintenance, Chromatin Binding