RPA1 antibody (AA 369-616)

Catalog No. ABIN1833491

The Rabbit Polyclonal anti-RPA1 antibody has been validated for WB, IHC, ICC and IHC (p). It is suitable to detect RPA1 in samples from Human and Mouse.

Quick Overview for RPA1 antibody (AA 369-616) (ABIN1833491)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RPA1 Antibody

Binding Specificity: AA 369-616

Specificity: Human RPA70 / RPA1

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 369 and 616 of RPA70 (SwissProt P27694). Percent identity by BLAST analysis: Human (100%), Bovine (93%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.0, 10 % glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Keep as concentrated solution. Avoid multiple freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA70 / RPA1

Background: Name/Gene ID: RPA1

Synonyms: RPA1, HSSB, MST075, Replication factor A protein 1, Replication protein A1 (70kD), RF-A, RF-A protein 1, MSTP075, Replication protein A1, 70  kDa, RP-A, REPA1, RP-A p70, RPA70

Gene ID: 6117

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA