INCENP antibody (AA 369-583)

Catalog No. ABIN1844019

This anti-INCENP antibody is a Mouse Monoclonal antibody detecting INCENP in WB, FACS, ELISA, IHC and IF/ICC. Suitable for Human.

Quick Overview for INCENP antibody (AA 369-583) (ABIN1844019)

Target: INCENP (Inner Centromere Protein Antigens 135/155kDa (INCENP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This INCENP antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-INCENP Antibody

Binding Specificity: AA 369-583

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS with 0.05 % sodium azide and 0.5 % protein stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.

Target Details for INCENP

Target: INCENP (Inner Centromere Protein Antigens 135/155kDa (INCENP))

Alternative Name: INCENP

Background: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A, MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143), MCAK (MIM 604538), KID (MIM 603213), cytoplasmic dynein (e.g., MIM 600112), CliPs (e.g., MIM 179838), and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis.

Molecular Weight: 105.4 kDa

Gene ID: 3619

UniProt: Q9NQS7

Pathways: Cell Division Cycle