E2F1 antibody (AA 69-223)

Catalog No. ABIN1845545

The Mouse Monoclonal anti-E2F1 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect E2F1 in samples from Human and Mouse.

Quick Overview for E2F1 antibody (AA 69-223) (ABIN1845545)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-E2F1 Antibody

Binding Specificity: AA 69-223

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS with 0.05 % sodium azide and 0.5 % protein stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Molecular Weight: 46.9 kDa

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy