SMAD4 antibody (C-Term)

Catalog No. ABIN184619

The Goat Polyclonal anti-SMAD4 antibody has been validated for ELISA and IP. It is suitable to detect SMAD4 in samples from Human.

Quick Overview for SMAD4 antibody (C-Term) (ABIN184619)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-SMAD4 Antibody

Binding Specificity: C-Term

Purpose: SMAD4 / MADH4

Sequence: HTMPIADPQP LD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HTMPIADPQPLD, from the C Terminus of the protein sequence according to NP_005350.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave a band at approx 75 kDa in lysates of cell line NIH3T3. Please note that currently we cannot find an explanation in the literature

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: MADH4, SMAD4, JIP, DPC4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against decapentaplegic, Drosophila, homolog of, 4, MAD (mothers against decapentaplegic, Drosophila) homolog 4, SMAD family member 4, OTTHUMP00000163548, SMAD,

Gene ID: 4089, 50554

NCBI Accession: NP_005350

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy