NFIL3 antibody (C-Term)

Catalog No. ABIN184644

This anti-NFIL3 antibody is a Goat Polyclonal antibody detecting NFIL3 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for NFIL3 antibody (C-Term) (ABIN184644)

Target: NFIL3 (Nuclear Factor, Interleukin 3 Regulated (NFIL3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NFIL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-NFIL3 Antibody

Binding Specificity: C-Term

Purpose: NFIL3

Sequence: RLIATQPISA SDSG

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RLIATQPISASDSG, from the C Terminus of the protein sequence according to NP_005375.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 60 kDa band observed in nuclear lysates of cell line Jurkat and HeLa (calculated MW of 51.5 kDa according to NP_005375.2). This molecular weight is routinely observed by other sources. An additional band of 30 kDa was observed in lysat

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NFIL3

Target: NFIL3 (Nuclear Factor, Interleukin 3 Regulated (NFIL3))

Alternative Name: NFIL3

Background: NFIL3, nuclear factor, interleukin 3 regulated, E4BP4, IL3BP1, NFIL3A, NF-IL3A, interleukin-3 promoter transcriptional activator, OTTHUMP00000021633

Gene ID: 4783, 18030, 114519

NCBI Accession: NP_005375