ATF4 antibody (C-Term)

Catalog No. ABIN184656

The Goat Polyclonal anti-ATF4 antibody (ABIN184656) specifically detects ATF4 in WB and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for ATF4 antibody (C-Term) (ABIN184656)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ATF4 Antibody

Binding Specificity: C-Term

Purpose: ATF4

Sequence: EEVRKARGKK RVP

Specificity: This antibody is expected to recognize both reported isoforms (NP_001666.2, NP_877962.1).

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EEVRKARGKKRVP, from the C Terminus of the protein sequence according to NP_001666.2, NP_877962.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35-37 kDa band observed in Mouse Heart lysates (calculated MW of 38.3 kDa according to Mouse NP_001274109.1, NP_033846.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background: ATF4, activating transcription factor 4 (tax-responsive enhancer element B67), CREB2, TXREB, CREB-2, TAXREB67, OTTHUMP00000199130, activating transcription factor 4, cAMP response element-binding protein 2

Gene ID: 468, 11911, 79255

NCBI Accession: NP_001666, NP_877962

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response