Cortactin antibody (N-Term)

Catalog No. ABIN184659

This anti-Cortactin antibody is a Goat Polyclonal antibody detecting Cortactin in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Cortactin antibody (N-Term) (ABIN184659)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Cortactin Antibody

Binding Specificity: N-Term

Purpose: Cortactin / EMS1

Sequence: WKASAGHAVS IA

Specificity: This antibody is expected to recognize both reported isoforms (NP_005222.2, NP_612632.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence WKASAGHAVSIA-C, from the N Terminus of the protein sequence according to NP_005222.2, NP_612632.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 80 kDa band observed in Human, Mouse and Rat Brain lysates and in lysates of cell line HEK293 (calculated MW of 61.6 kDa according to Human NP_005222.2 and of 61.2 kDa according to Mouse NP_031829.2). The observed molecular weight corr

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: CTTN

Background: EMS1, CORTACTIN, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate), Cttn, oncogene EMS1, CTTN, FLJ34459, 1110020L01Rik

Gene ID: 2017, 13043

NCBI Accession: NP_005222, NP_612632

Pathways: MAPK Signaling