CARD16 antibody (C-Term)

Catalog No. ABIN184661

The Goat Polyclonal anti-CARD16 antibody has been validated for ELISA, FACS and IF. It is suitable to detect CARD16 in samples from Human.

Quick Overview for CARD16 antibody (C-Term) (ABIN184661)

Target: CARD16 (Caspase Recruitment Domain Family, Member 16 (CARD16))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CARD16 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-CARD16 Antibody

Binding Specificity: C-Term

Purpose: COP1 / PSEUDO-ICE (Isofom 2)

Sequence: ETLGLSAGPI PGN

Specificity: This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068, NP_001007233.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ETLGLSAGPIPGN, from the C Terminus of the protein sequence according to NP_443121.1.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CARD16

Target: CARD16 (Caspase Recruitment Domain Family, Member 16 (CARD16))

Alternative Name: CARD16

Background: CARD16, COP, CARD only protein, PSEUDO-ICE, COP1, caspase-1 dominant-negative inhibitor pseudo-ICE, pseudo interleukin-1beta converting enzyme, caspase recruitment domain family, member 16

Gene ID: 114769

NCBI Accession: NP_443121