NOS1 antibody (C-Term)

Catalog No. ABIN184662

This anti-NOS1 antibody is a Goat Polyclonal antibody detecting NOS1 in IHC, ELISA, IF and FACS. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for NOS1 antibody (C-Term) (ABIN184662)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-NOS1 Antibody

Binding Specificity: C-Term

Purpose: NOS1

Sequence: ESKKDTDEVF SS

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Cortex. Recommended concentration: 6-8 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended con

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-NOS1 antibody (ABIN184662)

Fried, Kaupp, Müller: "Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice." in: Cell and tissue research, Vol. 339, Issue 3, pp. 463-79, (2010) (PubMed).

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1

Background: NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS, IHPS1, nNOS, nitric oxide synthase 1, neuronal

Gene ID: 4842

NCBI Accession: NP_000611, NP_001191147, NP_001191142

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction