Annexin A11 antibody (N-Term)

Catalog No. ABIN184685

The Goat Polyclonal anti-Annexin A11 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Annexin A11 in samples from Human.

Quick Overview for Annexin A11 antibody (N-Term) (ABIN184685)

Target: Annexin A11 (ANXA11)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Annexin A11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Annexin A11 Antibody

Binding Specificity: N-Term

Purpose: Annexin A11

Sequence: YPGYPPPPGG YPP

Specificity: Reported variants represent identical protein: NP_001148.1, NP_665875.1 and NP_665876.1.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence YPGYPPPPGGYPP-C, from the N Terminus of the protein sequence according to NP_001148.1, NP_665875.1, NP_665876.1

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of Basement Membrane in trophoblasts. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 55 kDa band observed in lysates of cell lines HeLa and Jurkat (calculated MW of 54.4 kDa according to NP_001148.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Annexin A11

Target: Annexin A11 (ANXA11)

Alternative Name: ANXA11

Background: ANXA11, annexin A11, ANX11, CAP50, annexin XI, autoantigen, 56-kD, calcyclin-associated annexin 50, RP11-369J21, OTTHUMP00000019957, OTTHUMP00000019958,

Gene ID: 311

NCBI Accession: NP_001148