BAK1 antibody (N-Term)

Catalog No. ABIN184699

This Goat Polyclonal antibody specifically detects BAK1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for BAK1 antibody (N-Term) (ABIN184699)

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-BAK1 Antibody

Binding Specificity: N-Term

Purpose: BAK1

Sequence: ASGQGPGPPR QE

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ASGQGPGPPRQE-C, from the N Terminus of the protein sequence according to NP_001179.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 25 kDa band observed in Human Adrenal Gland lysates and in preliminary testing of HeLa and Jurkat cell lyate (calculated MW of 23.4 kDa according to NP_001179.1. Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BAK1

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Alternative Name: BAK1

Background: BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer, BAK-LIKE, MGC117255, MGC3887, BCL2-like 7 protein, pro-apoptotic protein BAK

Gene ID: 578, 12018, 116502

NCBI Accession: NP_001179

Pathways: Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response