Cytohesin 1 Interacting Protein (CYTIP) (N-Term) antibody

Details for Product No. ABIN184711
Target
Binding Specificity
N-Term
9
4
1
1
1
Reactivity
Human
31
21
20
2
1
1
1
1
1
1
1
Host
Goat
27
6
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Western Blotting (WB)
20
13
8
4
2
1
1
Options
Purpose CYBR / PSCDBP
Immunogen Peptide with sequence SLQRLLQHSSNGN-C, from the N Terminus of the protein sequence according to NP_004279.3.
Blocking Peptide Blocking peptide for this product available: ABIN370458
Sequence SLQRLLQHSS NGN
Isotype IgG
Predicted Reactivity Human, Dog, Pig
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name CYTIP (CYTIP Antibody Abstract)
Background CYTIP, PSCDBP, CYBR, pleckstrin homology, Sec7 and coiled/coil domains, binding protein, HE, B3-1, CASP, cytohesin binding protein HE, cytohesin-1 interacting protein, pleckstrin homology, Sec7, and coiled-coil domains protein-binding protein, cytohesin 1 interacting protein, cytohesin binder and regulator, pleckstrin homology, Sec7 and coiled-coil domains, binding protein, pleckstrin homology, Sec7 and coiled/coil domains, binding protein
Gene ID 9595
NCBI Accession NP_004279
Application Notes

DS WB Results: Approx 38 kDa band observed in lysates of cell line Jurkat and approx 40 kDa in ysates of cell line MOLT4 (calculated MW of 40 kDa according to NP_004279.3). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment

Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of A431 and U251 cells. Recommended concentration: 10μg/ml.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-Cytohesin 1 Interacting Protein (CYTIP) (N-Term) antibody (ABIN184711) ABIN184711 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabi...
Image no. 2 for anti-Cytohesin 1 Interacting Protein (CYTIP) (N-Term) antibody (ABIN184711) ABIN184711 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per la...
Image no. 3 for anti-Cytohesin 1 Interacting Protein (CYTIP) (N-Term) antibody (ABIN184711) ABIN184711 (0.5µg/ml) staining of Jurkat (A) and (1ug/ml) MOLT4 (B) cell lysate (35µg...
Image no. 4 for anti-Cytohesin 1 Interacting Protein (CYTIP) (N-Term) antibody (ABIN184711) ABIN184711 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabi...