GRB7 antibody (N-Term)

Catalog No. ABIN184737

The Goat Polyclonal anti-GRB7 antibody (ABIN184737) specifically detects GRB7 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for GRB7 antibody (N-Term) (ABIN184737)

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRB7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GRB7 Antibody

Binding Specificity: N-Term

Purpose: GRB7 (N Terminus)

Sequence: ELDLSPPHLS SSPE

Specificity: This antibody recognises the longer isoform of this protein in A431 lysate.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ELDLSPPHLSSSPE-C, from the N Terminus of the protein sequence according to NP_005301.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65 kDa band observed in lysates of cell line A431 (calculated MW of 59.7 kDa according to NP_005301.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRB7

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Alternative Name: GRB7

Background: GRB7, growth factor receptor-bound protein 7, OTTHUMP00000164352

Gene ID: 2886, 14786

NCBI Accession: NP_005301

Pathways: EGFR Signaling Pathway, Ribonucleoprotein Complex Subunit Organization