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Iba1 antibody (C-Term)

Name: Iba1 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN184738
Price: 575.06 USD
Availability: InStock

This Goat Polyclonal antibody specifically detects Iba1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody. (Iba1 antibody (C-Term))

IHC

ABIN184738 (1µg/ml) staining of Human Frontal Cortex (A), Rat Brain (B), Mouse (C) Brain and Pig Brain (D) lysates (35µg protein in RIPA buffer). (Iba1 antibody (C-Term))

ABIN184738 (5µg/ml) staining of paraffin embedded Human Lung. (Iba1 antibody (C-Term))

IHC

ABIN184738 (1µg/ml) staining of Human Brain (Frontal Cortex (A) Rat (B) Mouse (C) Brain and Swine E) Brain lysates (35µg protein in RIPA buffer). (Iba1 antibody (C-Term))

ABIN184738 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. (Iba1 antibody (C-Term))

IHC

ABIN184738 (1µg/ml) staining of Human (A) and (0. (Iba1 antibody (C-Term))

ABIN184738 (2µg/ml) staining of paraffin embedded Mouse Brain. (Iba1 antibody (C-Term))

IHC

AIF1 / IBA1 staining of Rat Brain lysate (35 µg protein in RIPA buffer) by ABIN184738 (0. (Iba1 antibody (C-Term))

ABIN184738 (1µg/ml) staining of Human Frontal Cortex (A) and (0. (Iba1 antibody (C-Term))

ABIN184738 Negative Control showing staining of paraffin embedded Mouse Lung, with no primary antibody. (Iba1 antibody (C-Term))

IHC

ABIN184738 Staining of paraffin embedded Mouse Lung. (Iba1 antibody (C-Term))

IHC

ABIN184738 (1µg/ml) staining of Human Lymph node (A), Mouse Brain (B) and Rat Brain (C) lysate (35µg protein in RIPA buffer). (Iba1 antibody (C-Term))

ABIN184738 (1 μg/mL) staining of Mouse (A) and (2 μg/mL) Rat (B Brain lysate (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

ABIN184738 (6 μg/mL) staining of paraffin embedded Human Placenta. (Iba1 antibody (C-Term))

IHC

ABIN184738 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody. (Iba1 antibody (C-Term))

IHC

(ABIN184738) (1 μg/mL) staining of Mouse (A) and Rat (B) Brain lysate (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

(ABIN184738) (5 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

IHC

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex lysate (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

IHC

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Lung. (Iba1 antibody (C-Term))

IHC

(ABIN184738) (2 μg/mL) staining of Rat (A) and Mouse (B) Brain lysates (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

(ABIN184738) (4 μg/mL) staining of paraffin embedded Human Placenta. (Iba1 antibody (C-Term))

IHC

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysates (35 μg protein in RIPA buffer) Detected by chemiluminescence. (Iba1 antibody (C-Term))

IHC

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex (A) Mouse Brain (B) and Rat Brain (C) lysate (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

IHC

(ABIN184738) (4 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

IHC

41 images

Catalog No. ABIN184738

Quick Overview for Iba1 antibody (C-Term) (ABIN184738)

Target

See all Iba1 (IBA1) Antibodies

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity

Human, Rat, Mouse

Host

Goat

Clonality

Polyclonal

Conjugate

This Iba1 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade

Verified

Discover our top product IBA1 Primary Antibody

Binding Specificity
37

28

18

16

7

6

5

5

3

2

2

2

1

1

1
C-Term

Purpose

AIF1/IBA1 isoform 1 and 3

Sequence

TGPPAKKAIS ELP

Specificity

This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Cross-Reactivity

Human, Mouse, Pig, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen

Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3.

Isotype

IgG
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody
IBA1 Reactivity: Human WB, IHC, ICC, IF, IHC (p), FACS, IHC (fro), IHC (ff) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody
IBA1 Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 Reactivity: Human WB, IHC, IP, ICC, IF, IHC (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Rat WB Host: Mouse Monoclonal C5 unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (Internal Region) antibody
Verified IBA1 Reactivity: Human IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 35-102) antibody
IBA1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-146) antibody
IBA1 Reactivity: Human IHC, StM Host: Mouse Monoclonal AIF1-1909 unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 6-36), (N-Term) antibody
IBA1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB14652 unconjugated

Application Notes

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx. 16-17 kDa band observed in Human Frontal Cortex and in Mouse and Rat Brain lysates (calculated MW of 16.7 kDa according to Human NP_001614.3, 16.9 kDa according to Mouse NP_062340.1 and 16.8 kDa according to Rat NP_058892.1). Recommende
Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name

AIF1

Background

AIF1, IBLY409840A1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8

Gene ID

199, 11629, 29427

NCBI Accession

NP_116573, NP_001614

Pathways

Smooth Muscle Cell Migration