HLTF antibody (C-Term)

Catalog No. ABIN184752

This anti-HLTF antibody is a Goat Polyclonal antibody detecting HLTF in WB, ELISA and FACS. Suitable for Human.

Quick Overview for HLTF antibody (C-Term) (ABIN184752)

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HLTF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-HLTF Antibody

Binding Specificity: C-Term

Purpose: SMARCA3

Sequence: KQAKINEIRT LIDL

Specificity: .

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KQAKINEIRTLIDL, from the C Terminus of the protein sequence according to NP_003062.2, NP_620636.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 125 kDa band observed in nuclear lysates of cell line HeLa and in preliminary testing of A431 nuclear cell lysate (calculated MW of 114 kDa according to NP_003062.2). This molecular weight is routinely observed by other sources. Recom

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HLTF

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Alternative Name: HLTF

Background: HLTF, SMARCA3, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3, SNF2-like 3, sucrose nonfermenting-like 3, helicase-like transcription factor, DNA-binding prote

Gene ID: 6596, 20585

NCBI Accession: NP_003062, NP_620636