KLF15 antibody (N-Term)

Catalog No. ABIN184761

This anti-KLF15 antibody is a Goat Polyclonal antibody detecting KLF15 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for KLF15 antibody (N-Term) (ABIN184761)

Target: KLF15 (Kruppel-Like Factor 15 (KLF15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KLF15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-KLF15 Antibody

Binding Specificity: N-Term

Purpose: KLF15

Sequence: VDHLLPVDEN FSS

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence VDHLLPVDENFSS-C, from the N Terminus of the protein sequence according to NP_054798.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in HeLa and in nuclear cell lysates of HepG2 (calculated MW of 44 kDa according to NP_054798.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsucce

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KLF15

Target: KLF15 (Kruppel-Like Factor 15 (KLF15))

Alternative Name: KLF15

Background: KLF15, KKLF, Kruppel-like factor 15, KKLF protein, kidney-enriched Kruppel-like factor, DKFZp779M1320

Gene ID: 28999, 66277, 85497

NCBI Accession: NP_054798