KLF8 antibody (N-Term)

Catalog No. ABIN184763

The Goat Polyclonal anti-KLF8 antibody has been validated for WB, ELISA and IHC. It is suitable to detect KLF8 in samples from Human.

Quick Overview for KLF8 antibody (N-Term) (ABIN184763)

Target: KLF8 (Kruppel-Like Factor 8 (KLF8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KLF8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-KLF8 Antibody

Binding Specificity: N-Term

Purpose: KLF8 / BKLF3

Sequence: VDMDKLINNL EVQ

Specificity: This antibody is expected to recognise isoform 1 (NP_009181.2) and isoform 2 (NP_001152768.1).

Cross-Reactivity: Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence VDMDKLINNLEVQ-C, from the N Terminus of the protein sequence according to NP_009181.2, NP_001152768.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2) . In glioblastomas this protein was reported to run at appr. 60 kDa (Ding et al . J Biol Chem. 2005 Feb 25,280(8):6802-15, PMID: 15557280). Re

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KLF8

Target: KLF8 (Kruppel-Like Factor 8 (KLF8))

Alternative Name: KLF8

Background: KLF8, BKLF3, Kruppel-like factor 8, RP13-1021K9.1, DKFZp686O08126, DXS741, MGC138314, ZNF741, zinc finger protein 741, basic kruppel-like factor 3

Gene ID: 11279

NCBI Accession: NP_009181, NP_001152768