MLH3 antibody (C-Term)

Catalog No. ABIN184792

This Goat Polyclonal antibody specifically detects MLH3 in ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for MLH3 antibody (C-Term) (ABIN184792)

Target: MLH3 (MutL Homolog 3 (MLH3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MLH3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-MLH3 Antibody

Binding Specificity: C-Term

Purpose: MLH3

Sequence: RQSLQQSMPP CEPP

Specificity: This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RQSLQQSMPPCEPP, from the C Terminus of the protein sequence according to NP_001035197.1, NP_055196.2.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MLH3

Target: MLH3 (MutL Homolog 3 (MLH3))

Alternative Name: MLH3

Background: MLH3, mutL homolog 3 (E. coli), HNPCC, HNPCC7, MGC138372, S240II117, mutL (E. coli) homolog 3, mismatch repair gene MLH3, DNA mismatch repair protein MLH3, mutL homolog 3

Gene ID: 27030

NCBI Accession: NP_001035197, NP_055196

Pathways: Chromatin Binding