MLH3 antibody (AA 490-570)

Catalog No. ABIN7221088

This Rabbit Polyclonal antibody specifically detects MLH3 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for MLH3 antibody (AA 490-570) (ABIN7221088)

Target: MLH3 (MutL Homolog 3 (MLH3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLH3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MLH3 Antibody

Binding Specificity: AA 490-570

Purpose: Rabbit Anti-MLH3 Polyclonal Antibody

Specificity: MLH3 Polyclonal Antibody detects endogenous levels of MLH3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human MLH3 at AA range: 490-570

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MLH3

Target: MLH3 (MutL Homolog 3 (MLH3))

Alternative Name: MLH3

Background: MLH3, DNA mismatch repair protein Mlh3, MutL protein homolog 3MLH3 is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The mutL homolog 3 encoded by MLH3 functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Molecular Weight: 164kD

Gene ID: 27030

UniProt: Q9UHC1

Pathways: Chromatin Binding