AKR1A1 antibody (C-Term)

Catalog No. ABIN184795

This Goat Polyclonal antibody specifically detects AKR1A1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for AKR1A1 antibody (C-Term) (ABIN184795)

Target: AKR1A1 (Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKR1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-AKR1A1 Antibody

Binding Specificity: C-Term

Purpose: Aldehyde Reductase

Sequence: DAGHPLYPFN DPY

Specificity: Both reported variants (NP_006057.1and NP_697021.1) represent identical protein

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DAGHPLYPFNDPY, from the C Terminus of the protein sequence according to NP_006057.1, NP_697021.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Liver lysates (calculated MW of 36.6 kDa according to NP_006057.1 and NP_697021.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AKR1A1

Target: AKR1A1 (Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1))

Alternative Name: AKR1A1

Background: Aldehyde reductase, AKR1A1, aldo-keto reductase family 1, member A1 (aldehyde reductase), ALDR1, ALR, MGC12529, MGC1380, alcohol dehydrogenase, aldo-keto reductase family 1, member A1, DD3, OTTHUMP00000009240, dihydrodiol dehydrogenase 3

Gene ID: 10327, 58810, 78959

NCBI Accession: NP_006057, NP_697021

Pathways: Monocarboxylic Acid Catabolic Process