PAX5 antibody (N-Term)

Catalog No. ABIN184804

This Goat Polyclonal antibody specifically detects PAX5 in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PAX5 antibody (N-Term) (ABIN184804)

Target: PAX5 (Paired Box 5 (PAX5))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PAX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-PAX5 Antibody

Binding Specificity: N-Term

Purpose: PAX5 / BSAP

Sequence: DLEKNYPTPR TSR

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DLEKNYPTPRTSR-C, from the N Terminus of the protein sequence according to NP_057953.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in Human Lymph and Rat Spleen lysates, and approx.40 kDa in Mouse Spleen lysates (calculated MW of 42.1 kDa according to Human NP_057953.1 and 42.2 kDa according to Mouse NP_032808.1 and Rat NP_001102731.1). Recomme

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAX5

Target: PAX5 (Paired Box 5 (PAX5))

Alternative Name: PAX5

Background: PAX5, BSAP, paired box gene 5 (B-cell lineage specific activator protein), B-cell lineage specific activator protein, paired box homeotic gene 5 (B-cell lineage specific activator protein), paired box 5, B cell specific activator protein, B-cell lineage s

Gene ID: 5079, 18507

NCBI Accession: NP_057953