RNF31 antibody (C-Term)

Catalog No. ABIN184814

The Goat Polyclonal anti-RNF31 antibody has been validated for WB and ELISA. It is suitable to detect RNF31 in samples from Human.

Quick Overview for RNF31 antibody (C-Term) (ABIN184814)

Target: RNF31 (Ring Finger Protein 31 (RNF31))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RNF31 Antibody

Binding Specificity: C-Term

Purpose: RNF31 / ZIBRA (C terminus)

Sequence: EEVPLGQSIP RRRK

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EEVPLGQSIPRRRK, from the C Terminus of the protein sequence according to NP_060469.4.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 125 kDa band observed in lysates of cell line JurkatT(calculated MW of 120 kDa according to AAH12077.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RNF31

Target: RNF31 (Ring Finger Protein 31 (RNF31))

Alternative Name: RNF31

Background: RNF31, ZIBRA, FLJ10111, FLJ23501, zinc in-between-ring-finger ubiquitin-associated domain, ring finger protein 31, MGC19975, zinc in-between-RING-finger ubiquitin-associated domain protein

Gene ID: 55072

NCBI Accession: NP_060469