RPS27 antibody (N-Term)

Catalog No. ABIN184825

This Goat Polyclonal antibody specifically detects RPS27 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for RPS27 antibody (N-Term) (ABIN184825)

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RPS27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RPS27 Antibody

Binding Specificity: N-Term

Purpose: MPS1 / RPS27

Sequence: PLAKDLLHPS PEE

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PLAKDLLHPSPEE-C, from the N Terminus of the protein sequence according to NP_001021.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx. 12 kDa band observed in lysates of Human T-cell line Jurkat (calculated MW of 9.5 kDa according to NP_001021.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RPS27

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Alternative Name: RPS27

Background: MPS1, RPS27, ribosomal protein S27 (metallopanstimulin 1), MPS-1, metallopanstimulin 1, 40S ribosomal protein S27, RP11-422P24.3, metallopan-stimulin 1

Gene ID: 6232, 57294, 94266

NCBI Accession: NP_001021