RPS6KB1 antibody (C-Term)

Catalog No. ABIN184828

This Goat Polyclonal antibody specifically detects RPS6KB1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for RPS6KB1 antibody (C-Term) (ABIN184828)

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RPS6KB1 Antibody

Binding Specificity: C-Term

Purpose: p70S6K / RPS6KB1

Sequence: MISKRPEHLR MNL

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-MISKRPEHLRMNL, from the C Terminus of the protein sequence according to NP_003152.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in lysates of Human Placenta and of cell line NIH3T3 (calculated MW of 59.1 kDa according to NP_003152.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessfu

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: RPS6KB1

Background: RPS6KB1, p70-S6K, ribosomal protein S6 kinase, 70kD, polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, ribosomal protein S6 kinase, 70kD, polypeptide 1, serine/threonine kinase 14 alpha

Gene ID: 6198, 72508, 83840

NCBI Accession: NP_003152

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect