RAD51AP1 antibody (C-Term)

Catalog No. ABIN184863

This anti-RAD51AP1 antibody is a Goat Polyclonal antibody detecting RAD51AP1 in ELISA, IHC, IF and FACS. Suitable for Human.

Quick Overview for RAD51AP1 antibody (C-Term) (ABIN184863)

Target: RAD51AP1 (RAD51-Interacting Protein (RAD51AP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RAD51AP1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-RAD51AP1 Antibody

Binding Specificity: C-Term

Purpose: PIR51 / RAD51AP1

Sequence: ARVKPLHPNA TST

Specificity: This antibody is expected to recognise both reported isoforms.

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ARVKPLHPNATST, from the C Terminus of the protein sequence according to NP_001124334.1, NP_006470.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration: 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RAD51AP1

Target: RAD51AP1 (RAD51-Interacting Protein (RAD51AP1))

Alternative Name: RAD51AP1

Background: RAD51AP1, PIR51, RAD51-interacting protein, RAD51 associated protein 1

Gene ID: 10635

NCBI Accession: NP_001124334, NP_006470