RAD51AP1 antibody (C-Term)

Catalog No. ABIN184863

Product Details anti-RAD51AP1 Antibody

Target: RAD51AP1 (RAD51-Interacting Protein (RAD51AP1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RAD51AP1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: PIR51 / RAD51AP1

Sequence: ARVKPLHPNA TST

Specificity: This antibody is expected to recognise both reported isoforms.

Predicted Reactivity: Human, Dog, Cow

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ARVKPLHPNATST, from the C Terminus of the protein sequence according to NP_001124334.1, NP_006470.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Preliminary experiments gave an approx 25 kDa band in Human Testis lysates at 0.2 μg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 39kDda according to NP_006470. Have any further splice variants/modified forms been reported?

DS IHC Results: In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration, 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RAD51AP1

Target: RAD51AP1 (RAD51-Interacting Protein (RAD51AP1))

Alternative Name: RAD51AP1 (RAD51AP1 Products)

Synonyms: PIR51 antibody, 2510006L10Rik antibody, RAB22 antibody, RAD51 associated protein 1 antibody, Rad51ap1 antibody, RAD51AP1 antibody

Background: RAD51AP1, PIR51, RAD51-interacting protein, RAD51 associated protein 1

Gene ID: 10635

NCBI Accession: NP_001124334, NP_006470