PDIA3 antibody (C-Term)

Catalog No. ABIN184885

This Goat Polyclonal antibody specifically detects PDIA3 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PDIA3 antibody (C-Term) (ABIN184885)

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PDIA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PDIA3 Antibody

Binding Specificity: C-Term

Purpose: PDIA3 / ERp57 / GRP58

Sequence: EEKPKKKKKA QEDL

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EEKPKKKKKAQEDL, from the C Terminus of the protein sequence according to NP_005304.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in lysates of cell lines NIH3T3, HepG2 and Daudi (calculated MW of 56.8 kDa according to NP_005304.3). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was for 1 hour.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Alternative Name: PDIA3

Background: PDIA3, GRP58, ERp57, protein disulfide isomerase family A, member 3, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC, 58 kDa microsomal protein, endoplasmic reticulum P58, glucose regulated protein, 58 kDa, phospholipase C-alpha, protein disulfide isomerase-ass

Gene ID: 2923, 14827, 29468

NCBI Accession: NP_005304

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis