UBE2L3 antibody (C-Term)

Catalog No. ABIN184900

This Goat Polyclonal antibody specifically detects UBE2L3 in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse, Rat and Pig.

Quick Overview for UBE2L3 antibody (C-Term) (ABIN184900)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2L3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-UBE2L3 Antibody

Binding Specificity: C-Term

Purpose: UBE2L3

Sequence: EFTKKYGEKR PVD

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EFTKKYGEKRPVD, from the C Terminus of the protein sequence according to NP_003338.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 18 kDa band observed in lysates of cell lines HeLa HEK293, HepG2 and Jurkat and also in lysates of Human, Mouse and Rat Heart, Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9 kDa according to Human NP_003338.1 and Mo

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm. Dividing cells appear to show enhanced expression. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UBE2L3

Background: UBE2L3, ubiquitin-conjugating enzyme E2L 3, UBCH7, E2-F1, L-UBC, UbcM4, ubiquitin carrier protein, ubiquitin-conjugating enzyme UBCH7, ubiquitin-protein ligase

Gene ID: 7332, 22195

NCBI Accession: NP_003338