DPF1 antibody (C-Term)

Catalog No. ABIN184922

The Goat Polyclonal anti-DPF1 antibody has been validated for ELISA and IF. It is suitable to detect DPF1 in samples from Human.

Quick Overview for DPF1 antibody (C-Term) (ABIN184922)

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DPF1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-DPF1 Antibody

Binding Specificity: C-Term

Purpose: Neuro-d4 / DPF1

Sequence: HLKEKASAYI TLT

Specificity: This antibody is expected to recognize all three reported isoforms (NP_001128627.1, NP_004638.2, NP_001128628.1).

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HLKEKASAYITLT, from the C Terminus of the protein sequence according to NP_001128627.1, NP_004638.2, NP_001128628.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments showed a band at approx 38-40 kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1 μg/mL antibody staining (calculated MW of 37.9 kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DPF1

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Alternative Name: DPF1

Background: DPF1, NEUD4, neuro-d4, D4, zinc and double PHD fingers family 1, Neuro-d4 (rat) homolog, D4, zinc and double PHD fingers, family 1, MGC150428, MGC150429, BAF45b, OTTHUMP00000211615, neuro-d4 homolog

Gene ID: 8193, 29861, 50545

NCBI Accession: NP_001128627, NP_004638, NP_001128628