RNF8 antibody (N-Term)

Catalog No. ABIN184931

The Goat Polyclonal anti-RNF8 antibody has been validated for WB, ELISA and FACS. It is suitable to detect RNF8 in samples from Human.

Quick Overview for RNF8 antibody (N-Term) (ABIN184931)

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-RNF8 Antibody

Binding Specificity: N-Term

Purpose: RNF8

Sequence: GEPGFFVTGD RAG

Specificity: This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence GEPGFFVTGDRAG-C, from the N Terminus of the protein sequence according to NP_003949.1, NP_898901.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Testis and Lung lysates and approx. 50 kDa in nuclear lysates of cell lines HepG2 and Jurkat (predicted MW of 56 kDa according to NP_003949.1 and 50.8 kDa according to NP_898901.1 ). Recommended concentrati

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RNF8

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Alternative Name: RNF8

Background: RNF8, ring finger protein 8, ring finger protein (C3HC4 type) 8, KIAA0646, FLJ12013, C3HC4-type zinc finger protein, OTTHUMP00000039684, UBC13/UEV-interacting ring finger protein,

Gene ID: 9025

NCBI Accession: NP_003949, NP_898901

Pathways: Production of Molecular Mediator of Immune Response