TRIM8 antibody (C-Term)

Catalog No. ABIN184949

The Goat Polyclonal anti-TRIM8 antibody has been validated for ELISA, IHC, IF and FACS. It is suitable to detect TRIM8 in samples from Human.

Quick Overview for TRIM8 antibody (C-Term) (ABIN184949)

Target: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM8 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TRIM8 Antibody

Binding Specificity: C-Term

Purpose: GERP / TRIM8

Sequence: YGQPSTKHYV TS

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YGQPSTKHYVTS, from the C Terminus of the protein sequence according to NP_112174.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM8

Target: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Alternative Name: TRIM8

Background: TRIM8, tripartite motif-containing 8, GERP, RNF27, ring finger protein 27, tripartite motif protein TRIM8, glioblastoma expressed ring finger protein, RP11-47A8.4,

Gene ID: 81603, 93679

NCBI Accession: NP_112174