TRIM8 antibody (N-Term)

Catalog No. ABIN2778368

The Rabbit Polyclonal anti-TRIM8 antibody is suitable to detect TRIM8 in samples from Human, Rat, Mouse, Dog, Cow, Rabbit, Horse, Pig and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for TRIM8 antibody (N-Term) (ABIN2778368)

Target: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Reactivity: Human, Rat, Mouse, Dog, Cow, Rabbit, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TRIM8 Antibody

Binding Specificity: N-Term

Purpose: TRIM8 Antibody - N-terminal region

Sequence: RGCIGEAWAK DSGLVRCPEC NQAYNQKPGL EKNLKLTNIV EKFNALHVEK

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against TRIM8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRIM8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP39655-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TRIM8

Target: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Alternative Name: TRIM8

Background:

Background Information: TRIM8 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to nuclear bodies. Its structure is similar to some tumor suppressor proteins and its gene maps to a locus thought to contain tumor suppressor genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to nuclear bodies. Its structure is similar to some tumor suppressor proteins and its gene maps to a locus thought to contain tumor suppressor genes.

Gene Name: Tripartite motif containing 8

Alternative Symbols: GERP, RNF27

Protein Name: Probable E3 ubiquitin-protein ligase TRIM8

Molecular Weight: 61kDa

Gene ID: 81603

NCBI Accession: NP_112174

UniProt: Q9BZR9