USP20 antibody (C-Term)

Catalog No. ABIN185019

This anti-USP20 antibody is a Goat Polyclonal antibody detecting USP20 in ELISA, WB and IHC. Suitable for Human.

Quick Overview for USP20 antibody (C-Term) (ABIN185019)

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This USP20 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-USP20 Antibody

Binding Specificity: C-Term

Purpose: USP20 / VDU2

Sequence: HGEQKIEAET RAV

Specificity: Reported variants represent identical protein (NP_006667.3, NP_001103773.2, NP_001008563.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HGEQKIEAETRAV, from the C Terminus of the protein sequence according to NP_006667.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat). Recommended concentration, 3 μg/mL.

Western Blot: Approx 100 kDa band observed in Human Tonsil lysates (calculated MW of 102 kDa according to NP_006667.3). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for USP20

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Alternative Name: USP20

Background: USP20, VDU2, ubiquitin specific protease 20, KIAA1003, pVHL-interacting deubiquitinating enzyme 2, ubiquitin specific peptidase 20, LSFR3A

Gene ID: 10868, 74270

NCBI Accession: NP_006667

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling