Importin 9 antibody (C-Term)

Catalog No. ABIN185045

The Goat Polyclonal anti-Importin 9 antibody has been validated for ELISA, IHC and ICC. It is suitable to detect Importin 9 in samples from Human.

Quick Overview for Importin 9 antibody (C-Term) (ABIN185045)

Target: Importin 9 (IPO9)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Importin 9 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-Importin 9 Antibody

Binding Specificity: C-Term

Purpose: RANBPM / RANBP9

Sequence: GSCAFATVED YLH

Specificity: This product is also expected to recognize the centrosome-associated truncated isoform RANBPM (BAA23216.1).

Cross-Reactivity: Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence GSCAFATVEDYLH, from the C Terminus of the protein sequence according to NP_005484.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil stains nuclei in epithelial cells of tonsillar criptae. Recommended concentration: 3 μg/mL. Paraffin embedded Human Testis. Recommended concentration: 10 μg/mL.

Western Blot: Preliminary experiments gave no signal but low background in whole cell Hela lysates at up to 1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunocytochemistry: A customer reported successful use on HeLa.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Importin 9

Target: Importin 9 (IPO9)

Alternative Name: RANBP9

Background: RANBP9, RanBPM, RAN binding protein 9, RANBPM, novel centrosomal protein RanBPM, ran binding protein, centrosomal

Gene ID: 10048, 56705

NCBI Accession: NP_005484

Pathways: Protein targeting to Nucleus