PPP1R8 antibody (C-Term)

Catalog No. ABIN185050

This Goat Polyclonal antibody specifically detects PPP1R8 in WB, ELISA and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for PPP1R8 antibody (C-Term) (ABIN185050)

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP1R8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PPP1R8 Antibody

Binding Specificity: C-Term

Purpose: NIPP1 / PPP1R8

Sequence: EAWPGKKPTP SLLI

Specificity: This antibody is expected to recognise all three human isoforms of this protein.

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EAWPGKKPTPSLLI, from the C Terminus of the protein sequence according to NP_002704.1, NP_054829.2, NP_612568.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration: 2.5-3.8 μg/mL.

Western Blot: Approx 40 kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5 kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 h

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PPP1R8

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Alternative Name: PPP1R8

Background: PPP1R8, NIPP1, protein phosphatase 1, regulatory (inhibitor) subunit 8, ARD1, NIPP-1, PRO2047, ard-1, activator of RNA decay, nuclear subunit of PP-1, homolog of E.coli RNase E, nuclear inhibitor of protein phosphatase-1, protein phosphatase-1, regulatory

Gene ID: 5511, 100336

NCBI Accession: NP_002704, NP_054829, NP_612568