SNX5 antibody (C-Term)

Catalog No. ABIN185147

The Goat Polyclonal anti-SNX5 antibody has been validated for WB, ELISA and IHC. It is suitable to detect SNX5 in samples from Human.

Quick Overview for SNX5 antibody (C-Term) (ABIN185147)

Target: SNX5 (Sorting Nexin 5 (SNX5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SNX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SNX5 Antibody

Binding Specificity: C-Term

Purpose: SNX5

Sequence: SLLQSCIDLF KNN

Specificity: This antibody is expected to recognize both reported isoforms (NP_055241.1, NP_001269383.1). Reported variants represent identical protein: NP_055241.1, NP_689413.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SLLQSCIDLFKNN, from the C Terminus of the protein sequence according to NP_055241.1, NP_689413.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of Human Kidney and in trophoblasts of Human Placenta . Recommended concentration: 3-6 μg/mL.

Western Blot: Approx. 48 kDa band observed in Jurkat and A549 lysates (calculated MW of 46.8 kDa according to NP_055241.1). Recommended concentration: 1-3 μg/mL. Primary incubation was one hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SNX5

Target: SNX5 (Sorting Nexin 5 (SNX5))

Alternative Name: SNX5

Background: SNX5, FLJ10931, sorting nexin 5, RP11-504H3.2

Gene ID: 27131, 69178

NCBI Accession: NP_055241, NP_001269383