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PARD6A antibody (C-Term)

Verified PARD6A Reactivity: Human ELISA, WB, IHC, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185152
  • Target See all PARD6A Antibodies
    PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))
    Binding Specificity
    • 6
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 24
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 25
    • 2
    Goat
    Clonality
    • 27
    Polyclonal
    Conjugate
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PARD6A antibody is un-conjugated
    Application
    • 15
    • 12
    • 2
    • 2
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Purpose
    PAR6alpha / PARD6A
    Sequence
    GSRIRGDGSG FSL
    Specificity
    This antibody is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1, NP_001032358.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10 μg/mL. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocar

    Western Blot: Approx 40 kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4 kDa according to NP_058644.1) . Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Comment

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))
    Alternative Name
    PARD6A (PARD6A Products)
    Synonyms
    PARD6A antibody, 0710008C04Rik antibody, 2610010A15Rik antibody, PAR6alpha antibody, Par-6 antibody, Par6 antibody, Par6c antibody, TAX40 antibody, Tip-40 antibody, Par-6a antibody, Par6a antibody, PAR-6A antibody, PAR6 antibody, PAR6C antibody, TIP-40 antibody, par-6 partitioning defective 6 homolog alpha (C. elegans) antibody, par-6 family cell polarity regulator alpha antibody, Partitioning defective protein 6 antibody, PARD6A antibody, Pard6a antibody, par-6 antibody
    Background
    PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning d
    Gene ID
    50855
    NCBI Accession
    NP_058644, NP_001032358
    Pathways
    Cell-Cell Junction Organization
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