KPNA1 antibody (N-Term)

Catalog No. ABIN185156

The Goat Polyclonal anti-KPNA1 antibody has been validated for WB and ELISA. It is suitable to detect KPNA1 in samples from Human.

Quick Overview for KPNA1 antibody (N-Term) (ABIN185156)

Target: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KPNA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-KPNA1 Antibody

Binding Specificity: N-Term

Purpose: KPNA1 / Importin alpha 5

Sequence: TTPGKENFRL KSY

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TTPGKENFRLKSY-C, from the N Terminus of the protein sequence according to NP_002255.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 60.3 kDa according to NP_002255.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KPNA1

Target: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Alternative Name: KPNA1

Background: KPNA1, importin alpha 5, RCH2, SRP1, NPI-1, karyopherin alpha 1 (importin alpha 5), IPOA5, importin-alpha-S1, karyopherin alpha 1, nucleoprotein interactor 1, recombination activating gene cohort 2

Gene ID: 3836, 16646, 288064

NCBI Accession: NP_002255

Pathways: M Phase, Protein targeting to Nucleus