STK35 antibody (C-Term)

Catalog No. ABIN185178

This anti-STK35 antibody is a Goat Polyclonal antibody detecting STK35 in WB, ELISA, IHC, IF and FACS. Suitable for Human, Mouse and Pig. This Primary Antibody has been cited in 1 publication.

Quick Overview for STK35 antibody (C-Term) (ABIN185178)

Target: STK35 (Serine/threonine Kinase 35 (STK35))

Reactivity: Human, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This STK35 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-STK35 Antibody

Binding Specificity: C-Term

Purpose: Clik1 / STK35

Sequence: ELETRMDQVT CAA

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10 μg/mL.

Western Blot: Approx 55 kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58 kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1 kDa according to Human NP_543026.2, 59.5 kDa according to Mouse NP_899085.3

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-STK35 antibody (ABIN185178)

Goyal, Behring, Kumar, Siess: "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." in: PLoS ONE, Vol. 4, Issue 9, pp. e6981, (2009) (PubMed).

Target Details for STK35

Target: STK35 (Serine/threonine Kinase 35 (STK35))

Alternative Name: STK35

Background: STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form

Gene ID: 140901, 67333

NCBI Accession: NP_543026