DDX5 antibody (C-Term)

Catalog No. ABIN185199

This anti-DDX5 antibody is a Goat Polyclonal antibody detecting DDX5 in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for DDX5 antibody (C-Term) (ABIN185199)

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DDX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DDX5 Antibody

Binding Specificity: C-Term

Purpose: DDX5 / p68 RNA helicase

Sequence: PMIGYPMPTG YSQ

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PMIGYPMPTGYSQ, from the C Terminus of the protein sequence according to NP_004387.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in nuclear lysates of cell lines NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1 kDa according to NP_004387.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DDX5

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Alternative Name: DDX5

Background: DDX5, P68, HLR1, G17P1, HUMP68, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68 kDa), RNA-dependent ATPase, DEAD/H box-5 (RNA helicase, 68kD), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD), DEAD (Asp-Glu-Ala-Asp) box

Gene ID: 1655, 13207

NCBI Accession: NP_004387

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus