DDX5 antibody (C-Term)

Catalog No. ABIN2775191

This Rabbit Polyclonal antibody specifically detects DDX5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Rabbit. It has been mentioned in 2+ publications

Quick Overview for DDX5 antibody (C-Term) (ABIN2775191)

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX5 Antibody

Binding Specificity: C-Term

Purpose: DDX5 Antibody - C-terminal region

Sequence: SFGSNFVSAG IQTSFRTGNP TGTYQNGYDS TQQYGSNVPN MHNGMNQQAY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DDX5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DDX5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098629

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX5 antibody (ABIN2775191)

Huang, Chen, Wu, Huang, He, Tang, Wang, Wang: "The zebrafish miR-462/miR-731 cluster is induced under hypoxic stress via hypoxia-inducible factor 1α and functions in cellular adaptations." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 12, pp. 4901-13, (2015) (PubMed).

Naji, Ambrus, Cimerman?i?, Reyes, Johnson, Filbrandt, Huber, Vesely, Krogan, Yates, Saphire, Gerace: "Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 4, pp. M111.015313, (2012) (PubMed).

Target Details for DDX5

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Alternative Name: DDX5

Background:

Background Information: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX5 encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. DDX5 consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

Gene Name: DEAD (Asp-Glu-Ala-Asp) box helicase 5

Alternative Symbols: p68, HLR1, G17P1, HUMP68

Protein Name: Probable ATP-dependent RNA helicase DDX5

Molecular Weight: 68kDa

Gene ID: 1655

NCBI Accession: NP_004387

UniProt: P17844

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus