SMUG1 antibody (N-Term)

Catalog No. ABIN185204

The Goat Polyclonal anti-SMUG1 antibody has been validated for ELISA, IF and FACS. It is suitable to detect SMUG1 in samples from Human. There is 1 publication available.

Quick Overview for SMUG1 antibody (N-Term) (ABIN185204)

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SMUG1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SMUG1 Antibody

Binding Specificity: N-Term

Purpose: SMUG1

Sequence: PQAFLLGSIH EPA

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PQAFLLGSIHEPA-C, from the N Terminus of the protein sequence according to NP_055126.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 19 kDa in lysate of cell line HeLa after 0.1 μg/mL antibody staining (calculated MW of 19.6 kDa according to NP_001230718.1),. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U205 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SMUG1 antibody (ABIN185204)

Ma, Au, Waye: "Over-expression of SUMO-1 induces the up-regulation of heterogeneous nuclear ribonucleoprotein A2/B1 isoform B1 (hnRNP A2/B1 isoform B1) and uracil DNA glycosylase (UDG) in hepG2 cells." in: Cell biochemistry and function, Vol. 27, Issue 4, pp. 228-37, (2009) (PubMed).

Target Details for SMUG1

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Alternative Name: SMUG1

Background: SMUG1, single-strand selective monofunctional uracil DNA glycosylase, FDG, HMUDG, UNG3, single-strand-selective monofunctional uracil-DNA glycosylase 1, MGC104370

Gene ID: 23583

NCBI Accession: NP_055126

Pathways: DNA Damage Repair