Paired Box 3 antibody (N-Term)

Catalog No. ABIN185216

The Goat Polyclonal anti-Paired Box 3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect Paired Box 3 in samples from Human. There is 1 publication available.

Quick Overview for Paired Box 3 antibody (N-Term) (ABIN185216)

Target: Paired Box 3 (PAX3)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Paired Box 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Paired Box 3 Antibody

Binding Specificity: N-Term

Purpose: PAX3

Sequence: TTLAGAVPRM M

Specificity: This antibody is expected to recognize all 8 reported isoforms (NP_852122.1, NP_000429.2, NP_039230.1, NP_852123.1, NP_852124.1, NP_852126.1, NP_852125.1, NP_001120838.1).

Cross-Reactivity: Cow, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TTLAGAVPRMM-C, from the N Terminus of the protein sequence according to NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration: 4.5 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2 kDa according to NP_852125.1 and of 44.8 kDa according to NP_852126.1). Recommended concentration: 0.3-1 μg/mL. Primary in

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Paired Box 3 antibody (ABIN185216)

Hsieh, Yao, Lai, Yang: "Transcriptional repression activity of PAX3 is modulated by competition between corepressor KAP1 and heterochromatin protein 1." in: Biochemical and biophysical research communications, Vol. 349, Issue 2, pp. 573-81, (2006) (PubMed).

Target Details for Paired Box 3

Target: Paired Box 3 (PAX3)

Alternative Name: PAX3

Background: PAX3, WS1, CDHS, HUP2, paired box gene 3 (Waardenburg syndrome 1), paired domain gene 3, paired box homeotic gene 3 (Waardenburg syndrome 1), paired box 3, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, paired box homeotic gene 3, paired domain ge

Gene ID: 5077, 18505, 114502

NCBI Accession: NP_852122, NP_000429, NP_039230, NP_852123, NP_852124, NP_852126, NP_852125, NP_001120838

Pathways: Sensory Perception of Sound, Tube Formation