LIME antibody (C-Term)

Catalog No. ABIN185246

The Goat Polyclonal anti-LIME antibody has been validated for WB and ELISA. It is suitable to detect LIME in samples from Mouse.

Quick Overview for LIME antibody (C-Term) (ABIN185246)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This LIME antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-LIME Antibody

Binding Specificity: C-Term

Purpose: Lime1 (mouse)

Sequence: ENVYESIKEM GL

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-ENVYESIKEMGL

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 32 kDa band observed in Mouse Heart lysates (predicted MW of 31 kDa according to NP_076173.1). Recommended for use at 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Alternative Name: Lime1

Background: Lime1, Lck interacting transmembrane adaptor 1, 2810038K19Rik, LIME, RIKEN cDNA 2810038K19 gene, RP23-60M5.4, OTTMUSP00000018005

Gene ID: 72699, 362289

NCBI Accession: NP_076173