ANTXR1 antibody (C-Term)

Catalog No. ABIN185280

This anti-ANTXR1 antibody is a Goat Polyclonal antibody detecting ANTXR1 in ELISA and IF. Suitable for Human.

Quick Overview for ANTXR1 antibody (C-Term) (ABIN185280)

Target: ANTXR1 (anthrax Toxin Receptor 1 (ANTXR1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ANTXR1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ANTXR1 Antibody

Binding Specificity: C-Term

Purpose: TEM8 / Anthrax Toxin Receptor 1

Sequence: RAPPPSRPPP RPSV

Specificity: This antibody is expected to recognise variant 1 (NP_115584) only.

Cross-Reactivity: Cow, Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RAPPPSRPPPRPSV, from the C Terminus of the protein sequence according to NP_115584.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 65-70 kDa in lysate of cell lines A431 and U2OS after 1-2 μg/mL antibody staining (calculated MW of 62.8 kDa according to NP_115584.1). This band was successfully blocked by incubation with the immu

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membrane and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ANTXR1

Target: ANTXR1 (anthrax Toxin Receptor 1 (ANTXR1))

Alternative Name: ANTXR1

Background: ANTXR1, TEM8, anthrax toxin receptor, ATR, FLJ10601, FLJ21776, tumor endothelial marker 8, tumor endothelial marker 8, isoform 3 precursor, anthrax toxin receptor 1, FLJ11298, 2310008J16Rik, 2810405N18Rik

Gene ID: 84168

NCBI Accession: NP_115584