EHD2 antibody (C-Term)

Catalog No. ABIN185287

This Goat Polyclonal antibody specifically detects EHD2 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for EHD2 antibody (C-Term) (ABIN185287)

Target: EHD2 (EH-Domain Containing 2 (EHD2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EHD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-EHD2 Antibody

Binding Specificity: C-Term

Purpose: EHD2

Sequence: CRLVPPSKRR HKGSA

Specificity: This antibody is expected to recognise EHD1 protein as well as EHD2.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65-70 kDa band observed in Human Lung lysates and in lysates of cell lines A549 and HeLa (calculated MW of 61.2 kDa according to NP_055416.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. R

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EHD2

Target: EHD2 (EH-Domain Containing 2 (EHD2))

Alternative Name: EHD2

Background: EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617

Molecular Weight: 61.2kDa

Gene ID: 30846

NCBI Accession: NP_055416

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development