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EHD2 antibody (C-Term)

Verified EHD2 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185287
  • Target See all EHD2 Antibodies
    EHD2 (EH-Domain Containing 2 (EHD2))
    Binding Specificity
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 19
    • 8
    • 7
    Human
    Host
    • 16
    • 3
    Goat
    Clonality
    • 16
    • 3
    Polyclonal
    Conjugate
    • 13
    • 3
    • 2
    • 1
    This EHD2 antibody is un-conjugated
    Application
    • 15
    • 13
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    EHD2
    Sequence
    CRLVPPSKRR HKGSA
    Specificity
    This antibody is expected to recognise EHD1 protein as well as EHD2.
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx 65-70 kDa band observed in Human Lung lysates and in lysates of cell lines A549 and HeLa (calculated MW of 61.2 kDa according to NP_055416.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. R

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    EHD2 (EH-Domain Containing 2 (EHD2))
    Alternative Name
    EHD2 (EHD2 Products)
    Synonyms
    PAST2 antibody, BC027084 antibody, C130052H20Rik antibody, fj66d02 antibody, wu:fj66d02 antibody, zgc:77671 antibody, MGEPS antibody, MGC64266 antibody, EHD1 antibody, MGC107790 antibody, EH domain containing 2 antibody, EH-domain containing 2 antibody, EH-domain containing 2b antibody, EH domain containing 2 L homeolog antibody, EHD2 antibody, Ehd2 antibody, ehd2b antibody, ehd2.L antibody, ehd2 antibody
    Background
    EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617
    Molecular Weight
    61.2kDa
    Gene ID
    30846
    NCBI Accession
    NP_055416
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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